Stimulated emission double depletion (STEDD) nanoscopy with aberration compensation
Peng Gao & G. Ulrich Nienhaus (Institute of Applied Physics, Karlsruhe Institute of Technology)
Far-field optical fluorescence microscopy plays a key role in the discovery of biomolecular structure and dynamics, because it is minimally invasive and selective to specific biomolecules of interest. However, conventional optical microscopy has an ultimately spatial resolution about 200 nm, due to the well-known Abbe diffraction-limit. This talk will firstly introduce a super-resolution, background-free, 3D imaging technique, which is named as Stimulated Emission Double Depletion (STEDD) nanoscopy. Then, pupil-segmentation based aberration measurement will be introduced to further improve resolution and signal to noise (SNR) ratio of the STEDD image. In the end this talk will also show some applications of STEDD for biology.