Self-referencing digital holographic microscopy techniques for biological applications
Swapnil Mahajan (A,B) & Arun Anand (b)
a - Institut für Technische Optik, Universität Stuttgart, Germany
B - Optics Laboratory, Applied Physics Department, Faculty of Technology & Engineering, Maharaja Sayajirao University of Baroda, India
Digital holography is a quantitative phase contrast imaging technique which enables us to gain detailed information about an object under investigation. When combined with microscopy, digital holography leads to quantitative 3D imaging of micro objects with an added advantage of numerical focusing. Measurement of mechanical cell parameters is equally important with its physical properties to reveal the vigor and health of biological specimens. This can also lead to increase in the accuracy of disease detection. For measurement of mechanical parameters, imaging setup needs to be stable enough compared to cell thickness variations. The required stability is quite difficult to achieve with two beam digital holography setups. Here we present the development of common path, field portable and comparatively stable digital holographic microscopy techniques for quantification of temporal and physical parameters and their applications in the area of label-free 3D imaging, disease diagnosis and study of cell behavior.